Abstract
Two-phase aqueous systems have replaced and simplified steps in extraction and purification processes, especially for biocompounds. Thus, this study evaluated the liquid-liquid equilibrium behavior of Pluronic L64 + water + sodium salts at pH 5.0, 7.5, and 10.0. Sodium sulfate demonstrated the greatest phase separation, followed by sodium citrate and sodium tartrate. Higher pH values resulted in larger biphasic regions. The polymer distribution coefficients increased with the addition of salt. As pH increased, there was a tendency for Pluronic L64 to migrate to the polymer-rich phase. The Gibbs energy of micellization between −11,000 and −25,000 kJ mol−1 indicated the spontaneity of the process micellization for all systems, showing lower values for the systems with sodium sulfate. This parameter was related to the anion speciation at each pH. Besides, the effects of water structuring around ions and ion-polymer interaction influenced the phase separation.
Funder
Fundação de Amparo à Pesquisa do Estado de Minas Gerais—FAPEMIG
Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES
Partnerships Program for Education and Training (PAEC) between the Organization of American States (OEA) and the Coimbra Group of Brazilian Universities
Subject
Colloid and Surface Chemistry,Chemistry (miscellaneous)
Reference41 articles.
1. Poltronieri, P., and D’Urso, O.F. (2016). Biotransformation of Agricultural Waste and By-Products, Elsevier.
2. Biopharmaceutical benchmarks 2010;Walsh;Nat. Biotechnol.,2010
3. PEG–salt aqueous two-phase systems: An attractive and versatile liquid–liquid extraction technology for the downstream processing of proteins and enzymes;Glyk;Appl. Microbiol. Biotechnol.,2015
4. Liquid–liquid extraction of biopharmaceuticals from fermented broth: Trends and future prospects;Santos;J. Chem. Technol. Biotechnol.,2018
5. Rito-Palomares, M., and Benavides, J. (2017). Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products, Springer International Publishing.