The Placenta of Physcomitrium patens: Transfer Cell Wall Polymers Compared across the Three Bryophyte Groups

Abstract

Following similar studies of cell wall constituents in the placenta of Phaeoceros and Marchantia, we conducted immunogold labeling TEM studies of Physcomitrium patens to determine the composition of cell wall polymers in transfer cells on both sides of the placenta. Sixteen monoclonal antibodies were used to localize cell wall epitopes in the basal walls and wall ingrowths in this moss. In general, placental transfer cell walls of P. patens contained fewer pectins and far fewer arabinogalactan proteins AGPs than those of the hornwort and liverwort. P. patens also lacked the differential labeling that is pronounced between generations in the other bryophytes. In contrast, transfer cell walls on either side of the placenta of P. patens were relatively similar in composition, with slight variation in homogalacturonan HG pectins. Compositional similarities between wall ingrowths and primary cell walls in P. patens suggest that wall ingrowths may simply be extensions of the primary cell wall. Considerable variability in occurrence, abundance, and types of polymers among the three bryophytes and between the two generations suggested that similarity in function and morphology of cell walls does not require a common cell wall composition. We propose that the specific developmental and life history traits of these plants may provide even more important clues in understanding the basis for these differences. This study significantly builds on our knowledge of cell wall composition in bryophytes in general and in transfer cells across plants.