Rapid In Situ Biomonitoring of Subsoil Contamination by Applying an Algae-Soaked Disc Seeding Assay

Abstract

Various pollutants are pervasive in soil environments due to human activities, thereby damaging soil ecosystems. In this study, extension of a previously developed algae-soaked disc seeding assay for periodic evaluation of subsoil contamination over time was described. The assay can be used in different contamination configurations of silver nanoparticles in combination with examination of cell morphology, esterase activity, oxidative stress, and membrane permeability. In addition, we periodically attempted to repeat the algae-soaked disc seeding assay every three weeks. We evaluated applicability of this algae-soaked disc seeding assay using alga Chlamydomonas reinhardtii exposed to heterogeneous silver nanoparticle-contaminated soils. The results demonstrated that this assay is applicable for monitoring a change of subsoil contamination by periodic evaluation over time. The developed assay was identified as a periodically rapid in situ biomonitoring technique to measure subsoil contamination over time.