New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
-
Published:2021-07-01
Issue:13
Volume:22
Page:7098
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Ligasová AnnaORCID,
Koberna Karel
Abstract
Cytocentrifugation is a common technique for the capture of cells on microscopic slides. It usually requires a special cytocentrifuge or cytorotor and cassettes. In the study presented here, we tested the new concept of cytocentrifugation based on the threaded connection of the lid and the sample holder to ensure an adjustable flow of solutions through the filters and the collection of the filtered solutions in the reservoir during centrifugation. To test this concept, we developed a device for the preparation of cell samples on circular coverslips. The device was tested for the capture and sample processing of both eukaryotic and prokaryotic cells, cell nuclei, and mitochondria for microscopy analysis including image cytometry. Moreover, an efficient procedure was developed for capturing formaldehyde-fixed cells on non-treated coverslips without cell drying. The results showed that the tested arrangement enables the effective capture and processing of all of the tested samples and the developed device represents an inexpensive alternative to common cytocentrifuges, as only the paper filter is consumed during sample processing, and no special centrifuge, cytorotor, or cassette is necessary. As no additional system of solution removal is required during sample staining, the tested concept also facilitates the eventual automation of the staining procedure.
Funder
Technologická Agentura České Republiky
Ministerstvo Školství, Mládeže a Tělovýchovy
European Regional Development Fund
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference28 articles.
1. Een toestel voor cytologisch onderzoek van de liquor cerebrospinalis;Bots;Ned. T. Geneeskd,1963
2. A device for preparing cell spreads;Dore;Immunology,1965
3. A slide centrifuge: An apparatus for concentrating cells in suspension onto a microscope slide;Watson;J. Lab. Clin. Med.,1966
4. Principles of Cytocentrifugation
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献