Lipoprotein Particles as Shuttles for Hydrophilic Cargo-Reference-Cited by-同舟云学术

Lipoprotein Particles as Shuttles for Hydrophilic Cargo

Published:2023-04-28 Issue:5 Volume:13 Page:471
ISSN:2077-0375
Container-title:Membranes
language:en
Short-container-title:Membranes

Author:

Weber Florian¹²^ORCID,Axmann Markus¹^ORCID,Horner Andreas³^ORCID,Schwarzinger Bettina⁴,Weghuber Julian⁴⁵^ORCID,Plochberger Birgit¹^ORCID

Affiliation:

1. Department of Medical Engineering, University of Applied Sciences Upper Austria, 4020 Linz, Austria

2. Science for Life Laboratory, Department of Women’s and Children’s Health, Karolinska Institutet, 17164 Solna, Sweden

3. Institute of Biophysics, Johannes Kepler Universität, 4040 Linz, Austria

4. FFoQSI—Austrian Competence Centre for Feed and Food Quality, Safety & Innovation, 4600 Wels, Austria

5. Center of Excellence Food Technology and Nutrition, University of Applied Sciences Upper Austria, 4600 Wels, Austria

Abstract

Lipoprotein particles (LPs) are excellent transporters and have been intensively studied in cardiovascular diseases, especially regarding parameters such as their class distribution and accumulation, site-specific delivery, cellular internalization, and escape from endo/lysosomal compartments. The aim of the present work is the hydrophilic cargo loading of LPs. As an exemplary proof-of-principle showcase, the glucose metabolism-regulating hormone, insulin, was successfully incorporated into high-density lipoprotein (HDL) particles. The incorporation was studied and verified to be successful using Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM). Single-molecule-sensitive FM together with confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).

Funder

Federal State of Upper Austria, the “LandOÖ Basisfinanzierung”

Austrian Science Fund Project

Christian Doppler Forschungsgesellschaft

research project of the Austrian Competence Centre for Feed and Food Quality, Safety and Innovation

Austrian Research Promotion Agency

Publisher

MDPI AG

Subject

Filtration and Separation,Chemical Engineering (miscellaneous),Process Chemistry and Technology

Link

https://www.mdpi.com/2077-0375/13/5/471/pdf

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