Bioselective PES Membranes Based on Chitosan Functionalization and Virus-Imprinted NanoMIPs for Highly Efficient Separation of Human Pathogenic Viruses from Water

Abstract

Waterborne viruses are a public health concern due to relatively small infection doses. Particularly, adenoviruses (AdVs) are more resistant than RNA viruses to water purification treatments in terms of ultraviolet (UV) irradiation, pH, and chlorination tolerance. Moreover, AdVs are one of the most predominant waterborne viruses. Membrane separations have proven superior removal capabilities of waterborne pathogens over other separation methods. However, virus removal at ultratrace levels is still a significant challenge for current membrane technology. This study successfully addressed this challenge by developing a bioselective polyethersulfone (PES) membrane by a joint strategy involving chitosan hydrophilic surface modification and the immobilization of adenovirus-specific molecularly imprinted nanoparticles (nanoMIPs). The topological and chemical changes taking place on the membrane surface were characterized by using atomic force microscopy (AFM) and scanning electron microscopy (SEM). Furthermore, hydrophilicity and membrane performance were investigated in terms of swelling behavior, permeation flux, and surface fouling studies. The membrane efficacy was evaluated by filtration experiments, where the virus concentration of the loading solution before filtration and the permeates after filtration was quantified. The novel bioselective membrane showed excellent virus removal capabilities by separating 99.99% of the viruses from the water samples.