Simple Bioparticle Filtration Device Based on an Ultralow-Fouling Zwitterionic Polyurethane Membrane for Rapid Large-Volume Separation of Plasma and Viruses from Whole Blood
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Published:2023-05-17
Issue:5
Volume:13
Page:524
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ISSN:2077-0375
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Container-title:Membranes
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language:en
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Short-container-title:Membranes
Author:
Wang Kun1, Seol Hyang1, Cheng Alex12, McKeague Nash13, Carlson Megan1ORCID, Degraff Wade1, Huang Sijia4, Kim Sangil1ORCID
Affiliation:
1. Department of Chemical Engineering, University of Illinois at Chicago, Chicago, IL 60607, USA 2. New Trier High School, New Trier, IL 60093, USA 3. University of Chicago Laboratory Schools, Chicago, IL 60637, USA 4. Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
Abstract
Plasma separation from whole blood is oftent required as an essential first step when performing blood tests with a viral assay. However, developing a point-of-care plasma extraction device with a large output and high virus recovery remains a significant obstacle to the success of on-site viral load tests. Here, we report a portable, easy-to-use, cost-efficient, membrane-filtration-based plasma separation device that enables rapid large-volume plasma extraction from whole blood, designed for point-of-care virus assays. The plasma separation is realized by a low-fouling zwitterionic polyurethane-modified cellulose acetate (PCBU-CA) membrane. The zwitterionic coating on the cellulose acetate membrane can decrease surface protein adsorption by 60% and increase plasma permeation by 46% compared with a pristine membrane. The PCBU-CA membrane, with its ultralow-fouling properties, enables rapid plasma separation. The device can yield a total of 1.33 mL plasma from 10 mL whole blood in 10 min. The extracted plasma is cell-free and exhibits a low hemoglobin level. In addition, our device demonstrated a 57.8% T7 phage recovery in the separated plasma. The results of real-time polymerase chain reaction analysis confirmed that the nucleic acid amplification curve of the plasma extracted by our device is comparable to that obtained by centrifugation. With its high plasma yield and good phage recovery, our plasma separation device provides an excellent replacement for traditional plasma separation protocols for point-of-care virus assays and a broad spectrum of clinical tests.
Funder
University of Illinois, Chicago (UIC)’s Chancellor’s Translational Research Initiative program Lawrence Livermore National Laboratory’s Lab Directed Research and Development program
Subject
Filtration and Separation,Chemical Engineering (miscellaneous),Process Chemistry and Technology
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