Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21

Author:

Cantini Francesca12ORCID,Giannì Paola34,Bobone Sara3,Troiano Cassandra3,van Ingen Hugo5ORCID,Massoud Renato6,Perini Nicoletta7,Migliore Luciana78ORCID,Savarin Philippe9ORCID,Sanders Charles101112,Stella Lorenzo3ORCID,Sette Marco39ORCID

Affiliation:

1. Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino, 50019 Firenze, Italy

2. Department of Chemistry, University of Florence, Sesto Fiorentino, 50019 Firenze, Italy

3. Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy

4. Fondazione G.I.M.EM.A.-Franco Mandelli Onlus, 00182 Rome, Italy

5. NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

6. Department of Experimental Medicine, University of Rome Tor Vergata, Viale della Ricerca Scientifica, 00133 Rome, Italy

7. Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy

8. eCampus University, 22060 Novedrate, Italy

9. Chemistry Structures Properties of Biomaterials and Therapeutic Agents Laboratory (CSPBAT), Nanomédecine Biomarqueurs Détection Team (NBD), Sorbonne Paris Nord University, The National Center for Scientific Research (CNRS), UMR 7244, 74 Rue Marcel Cachin, CEDEX, 93017 Bobigny, France

10. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA

11. Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA

12. Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA

Abstract

(1) Background: antimicrobial resistance is becoming a dramatic problem for public health, and the design of new antimicrobial agents is an active research area. (2) Methods: based on our previous work, we designed an improved version of the crabrolin peptide and characterized its functional and structural properties with a wide range of techniques. (3) Results: the newly designed peptide, crabrolin21, is much more active than the previous ones and shows specific selectivity towards bacterial cells. (4) Conclusions: crabrolin21 shows interesting properties and deserves further studies.

Funder

iNEXT-Discovery in Horizon 2020

Italian Ministry of University and Research

Regione Lazio

Publisher

MDPI AG

Subject

Filtration and Separation,Chemical Engineering (miscellaneous),Process Chemistry and Technology

Reference37 articles.

1. Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis;Murray;Lancet,2022

2. Increased multi-drug resistant Escherichia coli from hospitals in Khartoum state, Sudan;Ibrahim;Afr. Health Sci.,2013

3. Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review;Mulani;Front. Microbiol.,2019

4. Host defense peptides and their antimicrobial-immunomodulatory duality;Steinstraesser;Immunobiology,2011

5. Multifunctional cationic host defence peptides and their clinical applications;Yeung;Cell. Mol. Life Sci.,2011

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