Affiliation:
1. College of Food Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, China
2. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3. Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350012, China
Abstract
Microbial xylanase has wide application in bioenergy, animal feed, environmental protection, the pulp and paper industry, and agricultural development. In this study, three xylanases from the microbiota of T. fuciformis with its companion strains were identified by metagenomics sequencing. The three enzymes were subjected to cloning and expression in E. coli or P. pastoris, purification, and characterization for their properties. The results showed that AsXyn1, from Annulohypoxylon stygium, among the three enzymes possessed high thermostability at 40 °C and broad pH tolerance in the range of 2.0–10.0, exhibiting its application potential. Furthermore, it was found that post-translational modification (such as glycosylation) of AsXyn1 enzyme modulated its activity, kinetic parameters, and thermostability. These results and findings provided a hint for enzyme modification and design in future.
Funder
Fujian Science and technology support program
Subject
Physical and Theoretical Chemistry,Catalysis,General Environmental Science
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