Mitigation of Copper Stress in Maize by Inoculation with Paenibacillus polymyxa and Bacillus circulans

Abstract

Copper (Cu) is a micronutrient that assumes a principal role in plant growth and development. However, its excess concentration in soil is imperiling crop productivity. Inoculation with different bacterial strains in cereals could modify growth traits, photosynthetic effectiveness, and generation of strong antioxidant defense systems to make them more tolerant of Cu stress. Therefore, a pot study was designed to test plant growth-promoting rhizobacteria (PGPR) including Paenibacillus polymyxa and Bacillus circulans to Cu exposed maize (Zea mays L.) plants. Increasing Cu (100 to 500 µM of CuSO4) concentration decreased growth traits, photosynthetic pigments, soluble sugars, phosphorous (P) and potassium (K) contents, and the activity of catalase (CAT) but increased proline and malondialdehyde (MDA) content, the activity of peroxidase (POD) and Cu ions at root and shoot level. Moreover, the bacterial treatment also modulated the antioxidant capability in stress-free plants. Nevertheless, inoculation with P. polymyxa and B. circulans alleviated Cu-induced growth, photosynthetic pigments and mineral nutrient (P and K) on one hand and regulating the pools of osmolytes and antioxidant enzymes, whilst simultaneously reducing MDA and Cu root and shoot contents. These improved activities of antioxidant enzymes and the regulation of osmolytes content elicited by the blend of bacterial inoculation would have retained the ability of maize plants to confer resilience to Cu stress. This study further affirms that the application of two specific bacterial strains to maize plants proved very effective to ameliorate the Cu toxicity.