Comparative Genome Analysis of Two Streptococcus suis Serotype 8 Strains Identifies Two New Virulence-Associated Genes
Author:
Sheng Qi123, Xu Qiuhua123, Lan Zouran14, Wu Zongfu1235ORCID
Affiliation:
1. MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China 2. Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210014, China 3. WOAH Reference Lab for Swine Streptococcosis, Nanjing 210014, China 4. Shandong Provincial Center for Animal Disease Control, Jinan 250100, China 5. Guangdong Provincial Key Laboratory of Research on the Technology of Pig-Breeding and Pig-Disease Prevention, Guangzhou 511400, China
Abstract
Streptococcus suis is an important zoonotic pathogen that can cause meningitis and septicemia in swine and humans. Among numerous pathogenic serotypes, S. suis serotype 8 has distinctive characteristics such as a high detection rate and causing multi-host infection. There is no complete genome of serotype 8 strains so far. In this study, the complete genome of two S. suis serotype 8 strains, virulent strain 2018WUSS151 and non-virulent strain WUSS030, were sequenced. Comparative genomic analysis showed that the homology of the two genomes reaches 99.68%, and the main difference is the distinctive prophages. There are 83 genes unique to virulent strain 2018WUSS151, including three putative virulence-associated genes (PVGs). Two PVGs, padR and marR, are passenger genes in ISSsu2 family transposons that are able to form circular DNA intermediates during transposition, indicating the possibility of horizontal transmission among S. suis strains. The deletion mutant of PVGs marR or atpase attenuated the virulence of serotype 2 virulent SC070731 in a mouse infection model, confirming their role in S. suis virulence. These findings contribute to clarifying the genomic characterization of S. suis serotype 8 and S. suis pathogenesis.
Funder
National Key Research and Development Program of China National Natural Science Foundation of China Open Project Program of Jiangsu Key Laboratory of Zoonosis Open Project Program of Engineering Research Center for the Prevention and Control of Animal Original Zoonosis, Fujian Province University
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