Affiliation:
1. Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró 59625-900, Brazil
2. Smithsonian National Zoo and Conservation Biology Institute, Washington, DC 20008, USA
Abstract
The objective was to characterize morphological, morphometric, and ultrastructural changes in rhea spermatozoa between the epididymis and the vas deferens. Sperm samples were collected from the reproductive tracts of seven adult individuals and evaluated for sperm characteristics using brightfield microscopy as well as ultrastructural features using scanning electron microscopy (SM). Mean sperm count tended to increase in the vas deferens (378.0 ± 135.0 × 106) compared to the epididymis (201.0 ± 77.4 × 106). Percentages of motile sperm grew from 37.0 ± 4.9% in the epididymis to 58.5 ± 7.7% in the vas deferens. The proportion of normal spermatozoa was 75.6 ± 1.8% and most common defects were bent tails (9.7 ± 0.9%). However, these proportions were not different between epididymis and vas deferens. SM analysis revealed further features of rhea spermatozoa. Normal rhea spermatozoa were threadlike with an acrosome (0.95 ± 0.0 µm), head (7.53 ± 0.01 µm), midpiece (2.08 ± 0.01 µm), and tail (30.7 ± 0.06 µm). Lengths of sperm acrosome, head, midpiece, and tail were longer in the vas deferens compared to the epididymis. Our findings suggest that rhea spermatozoa undergo a maturation process during the passage from the epididymis to the vas deferens.
Funder
the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brazil
the National Council for the Scientific Development
Subject
General Veterinary,Animal Science and Zoology
Reference48 articles.
1. Effect of passage through the gut of Greater Rheas on the germination of seeds of plants of cerrado and caatinga grasslands;Azevedo;Emu,2013
2. BirdLife International (2016). The IUCN Red List of Threatened Species, Available online: https://www.iucnredlist.org/species/22678073/92754472.
3. Slaughtering yield and composition of great rhea meat (Rhea americana);Pereira;Food Sci. Technol.,2006
4. Viabilidade da exploração de ratitas em São Paulo;Giannoni;Biológico,1998
5. Challenges and current developments in the use of assisted reproduction techniques in wild birds;Pereira;Rev. Bras. Reprod. Anim.,2017
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