Affiliation:
1. School of Pharmacy, Hangzhou Normal University, Hangzhou 311121, China
2. Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou 311121, China
Abstract
β-Elemene (C15H24), a sesquiterpenoid compound isolated from the volatile oil of Curcuma wenyujin, has been proven to be effective for multiple cancers and is widely used in clinical treatment. Unfortunately, the β-elemene content in C. wenyujin is very low, which cannot meet market demands. Our previous research showed that methyl jasmonate (MeJA) induced the accumulation of β-elemene in C. wenyujin. However, the regulatory mechanism is unclear. In this study, 20 jasmonate ZIM-domain (JAZ) proteins in C. wenyujin were identified, which are the core regulatory factors of the JA signaling pathway. Then, the conservative domains, motifs composition, and evolutionary relationships of CwJAZs were analyzed comprehensively and systematically. The interaction analysis indicated that CwJAZs can form homodimers or heterodimers. Fifteen out of twenty CwJAZs were significantly induced via MeJA treatment. As the master switch of the JA signaling pathway, the CwMYC2-like protein has also been identified and demonstrated to interact with CwJAZ2/3/4/5/7/15/17/20. Further research found that the overexpression of the CwMYC2-like gene increased the accumulation of β-elemene in C. wenyujin leaves. Simultaneously, the expressions of HMGR, HMGS, DXS, DXR, MCT, HDS, HDR, and FPPS related to β-elemene biosynthesis were also up-regulated by the CwMYC2-like protein. These results indicate that CwJAZs and the CwMYC2-like protein respond to the JA signal to regulate the biosynthesis of β-elemene in C. wenyujin.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Zhejiang province
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference67 articles.
1. Study on ingredients of essential oils of Curcuma wenyujin extracted by supercritical-CO2 fluid extraction and steam distillation;Li;China J. Chin. Mater. Med.,2006
2. Recent advances in biosynthesis and pharmacology of β-elemene;Chen;Phytochem. Rev.,2023
3. Drug delivery systems for elemene, its main active ingredient beta-elemene, and its derivatives in cancer therapy;Zhai;Int. J. Nanomed.,2018
4. Metabolic engineering of Saccharomyces cerevisiae for production of germacrene A, a precursor of beta-elemene;Hu;J. Ind. Microbiol. Biotechnol.,2017
5. Improved production of germacrene A, a direct precursor of β-elemene, in engineered Saccharomyces cerevisiae by expressing a cyanobacterial germacrene A synthase;Zhang;Microb. Cell Fact.,2021