Technical Implications of the Chicken Embryo Chorioallantoic Membrane Assay to Elucidate Neuroblastoma Biology
-
Published:2023-09-29
Issue:19
Volume:24
Page:14744
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Patiño-Morales Carlos César12ORCID, Jaime-Cruz Ricardo13ORCID, Ramírez-Fuentes Tania Cristina14, Villavicencio-Guzmán Laura1, Salazar-García Marcela15ORCID
Affiliation:
1. Developmental Biology Research Laboratory, Hospital Infantil de México Federico Gómez, Mexico City 06720, Mexico 2. Cell Biology Laboratory, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico City 05348, Mexico 3. Department of Health Sciences, Universidad Tecnológica de México-UNITEC México-Campus Sur, Mexico City 09810, Mexico 4. Section of Graduate Studies and Research, School of Medicine of the National Polytechnic Institute, Mexico City 11340, Mexico 5. Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04360, Mexico
Abstract
The chorioallantoic membrane (CAM) can be used as a valuable research tool to examine tumors. The CAM can be used to investigate processes such as migration, invasion, and angiogenesis and to assess novel antitumor drugs. The CAM can be used to establish tumors in a straightforward, rapid, and cost-effective manner via xenotransplantation of cells or tumor tissues with reproducible results; furthermore, the use of the CAM adheres to the three “R” principle, i.e., replace, reduce, and refine. To achieve successful tumor establishment and survival, several technical aspects should be taken into consideration. The complexity and heterogeneity of diseases including neuroblastoma and cancers in general and their impact on human health highlight the importance of preclinical models that help us describe tumor-specific biological processes. These models will not only help in understanding tumor biology, but also allow clinicians to explore therapeutic alternatives that will improve current treatment strategies. In this review, we summarize the technical characteristics as well as the main findings regarding the use of this model to study neuroblastoma for angiogenesis, metastasis, drug sensitivity, and drug resistance.
Funder
Children’s Hospital of México Federico Gomez
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference101 articles.
1. Chen, L., Wang, S., Feng, Y., Zhang, J., Du, Y., Zhang, J., Van Ongeval, C., Ni, Y., and Li, Y. (2021). Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research. Cells, 10. 2. Chick embryo chorioallantoic membrane as a suitable in vivo model to evaluate drug delivery systems for cancer treatment: A review;Victorelli;Eur. J. Pharm. Biopharm.,2020 3. Ahmed, T.A.E., Cordeiro, C.M.M., Elebute, O., and Hincke, M.T. (2022). Proteomic Analysis of Chicken Chorioallantoic Membrane (CAM) during Embryonic Development Provides Functional Insight. BioMed Res. Int., 2022. 4. The chick embryo chorioallantoic membrane (CAM) assay;Ribatti;Reprod. Toxicol.,2017 5. Kundeková, B., Máčajová, M., Meta, M., Čavarga, I., and Bilčík, B. (2021). Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications. Biology, 10.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|