The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen-Reference-Cited by-同舟云学术

The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen

Published:2024-04-30 Issue:5 Volume:14 Page:579
ISSN:2075-1729
Container-title:Life
language:en
Short-container-title:Life

Author:

Ruszova Ema¹²^ORCID,Vanek Daniel¹²³⁴^ORCID,Stühmer Walter⁵^ORCID,Khaznadar Ziad¹,Subhashini Nidhi⁶^ORCID

Affiliation:

1. Bulovka University Hospital, 180 81 Prague, Czech Republic

2. Forensic DNA Service, Budinova 2, 180 81 Prague, Czech Republic

3. Institute for Environmental Sciences, Charles University, 110 00 Prague, Czech Republic

4. Department of Forensic Medicine, Second Faculty of Medicine, Charles University, 110 00 Prague, Czech Republic

5. Max Planck Institute of Multidisciplinary Sciences, Hermann-Rein-Str. 3, 37075 Göttingen, Germany

6. Seratec GmbH, Ernst-Ruhstrat-Str. 5, 37079 Göttingen, Germany

Abstract

Our research has developed a highly sensitive and simple assay to detect small amounts of animal and human biological material in less than 40 min. The handheld SaLux19 device developed at the Max Planck Institute of Experimental Medicine in Göttingen, Germany, was used to validate our concept. The proposed system uses isothermal amplification of DNA in a rapid assay format. Our results show that the assay can detect Sus scrofa nucleic acids with very high sensitivity and specificity. This detection system has potential for forensic scenarios.

Publisher

MDPI AG

Link

https://www.mdpi.com/2075-1729/14/5/579/pdf

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