Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

Author:

Zhang Qiang,Bai Yi,Chen Zhi,Mo Jiezhang,Tian Yulu,Guo Jiahua

Abstract

Lincomycin (LIN), as a waterborne contaminant, may pose a threat to algal health and may affect the provision of ecosystem services. In addition, the molecular mechanisms of lincomycin in algae are still unknown. Here, we attempted to use the transcriptome analysis to elucidate for the first time the potential impact of LIN at an environmentally relevant concentration on the algal growth, and verify the hypothesis that lincomycin can disrupt algal protein synthesis by combining with its subunits of ribosome at high-LIN level. In this study, 7-day growth inhibition tests and RNA-seq sequencing were conducted in Raphidocelis subcapitata (R. subcapitata) in response to a LIN at the concentrations of 0.5 µg L−1 (low), 5 µg L−1 (medium), and 400 µg L−1 (high) treatment groups. A negligible influence on algal growth and merely 21 (21 up- and 0 downregulated) differentially expressed genes (DEGs) was observed at low concentration of LIN, and medium groups showed a 13.4% inhibition and 92 (64 up- and 48 downregulated) DEGs, while high-LIN dosing caused 65.4% reduction in algal growth and 2514 (663 up- and 1851 downregulated) DEGs. In 0.5 and 5 µg L−1 groups, LIN upregulated the genes in the process of photosynthesis consisting of photosynthesis-antenna proteins, and porphyrin and chlorophyll metabolism pathways, suggesting that photosynthesis at low LIN exposure was more sensitive than algal growth. Whereas DEGs in the 400 µg L−1 group were mostly enriched in carbohydrate, carbon fixation in photosynthetic organisms, and nucleotide metabolism pathways. Furthermore, genes involved in detoxification processes were nearly downregulated in high-LIN group. In addition, genes encoding the antioxidant enzymes in the peroxisome pathway such as superoxide dismutase (sod2), peroxin-2 (pex2), 2,4-dienoyl-CoA reductase ((3E)-enoyl-CoA-producing) (decr2) were upregulated, which are responsible for deleting extra intracellular reactive oxygen species (ROS) caused by LIN to protect algal health, suggesting the occurrence of oxidative stress. Taken together, this is the first meticulous study unraveling the molecular mechanism of antibiotics in algae.

Publisher

MDPI AG

Subject

Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3