Affiliation:
1. Tsitsin Main Botanical Garden, Russian Academy of Sciences, 127276 Moscow, Russia
Abstract
Understanding the critical stages in ovule development is essential for optimizing in vitro seed propagation, cryopreservation, and indirect somatic plant embryogenesis. We studied the main stages of the megasporogenesis and megagametogenesis of Coelogyne speciosa subsp. fimbriata (J.J.Sm.) Gravendeel by confocal laser scanning microscopy. The ovule is anatropic, tenuinucellate, bitegmal, and funicular. The embryo sac is monosporic, Polygonum-type, bipolar, and usually 8-nuclear, but in cases of a reduction in the last mitotic division, it is 6-nuclear. Endosperm formation was not detected. The formation of a functional megaspore occurred 65–70 days after pollination (DAP), while the formation of a mature embryo sac and fertilization occurred after 85–100 DAP.
Funder
Tsitsin Main Botanical Garden, Russian Academy of Sciences
Reference34 articles.
1. An updated classification of Orchidaceae;Chase;Bot. J. Linn. Soc.,2015
2. The number of known plants species in the world and its annual increase;Christenhusz;Phytotaxa,2016
3. Coelogyne;Pridgeon;Genera Orchidacearum, Epidendroideae (Part One),2005
4. Alrich, P.W., and Higgins, W. (2008). The Marie Selby Botanical Gardens Illustrated Dictionary of Orchid Genera, Cornell University Press.
5. Revision of Coelogyne section Speciosae (Orchidaceae);Gravendeel;Blumea,1999