Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line-Reference-Cited by-同舟云学术

Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line

Published:2023-11-12 Issue:11 Volume:13 Page:1599
ISSN:2075-4426
Container-title:Journal of Personalized Medicine
language:en
Short-container-title:JPM

Author:

Kussainova Assiya¹²^ORCID,Bulgakova Olga²^ORCID,Aripova Akmaral²^ORCID,Ibragimova Milana²³^ORCID,Pulliero Alessandra¹^ORCID,Begimbetova Dinara³,Bersimbaev Rakhmetkazhi²,Izzotti Alberto⁴⁵^ORCID

Affiliation:

1. Department of Health Sciences, University of Genova, Via Pastore 1, 16132 Genoa, Italy

2. Department of General Biology and Genomics, Institute of Cell Biology and Biotechnology, L.N. Gumilyov Eurasian National University, Astana 010008, Kazakhstan

3. National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan

4. Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy

5. IRCCS Ospedale Policlinico San Martino, 16132 Genoa, Italy

Abstract

Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos.

Funder

Ministry of Science and Higher Education of the Republic of Kazakhstan

Italian Association for Cancer Research

Publisher

MDPI AG

Subject

Medicine (miscellaneous)

Link

https://www.mdpi.com/2075-4426/13/11/1599/pdf

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