Abstract
The balance between embolic risk and bleeding represents a clinical challenge in cancer patient treatment, encouraging studies on adjuvant oncologic treatments. Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation and the antineoplastic effect on melanoma cells (B16-F10) by applying platelet aggregation, angiogenesis and viability cell assays. The results displayed a significant platelet antiaggregant effect, corresponding to 50 and 80% for the extract and EGCG, respectively, compared to the negative control. Furthermore, both GTE and EGCG exhibited antitumor effects by reducing melanoma cell growth by 25 and 50%, respectively, verified by cellular apoptosis. Regarding angiogenesis, these substances inhibited blood vessel formation, reaching about 25% and 99% for GTE and EGCG at 100 μg/mL, respectively. Moreover, TNF-α cell stimulation evidenced VEGF and IL-8 secretion inhibition at 55 and 20% with GTE, while EGCG promoted an inhibition around 78% for both VEGF and IL-8. The results indicate the promising performance of GTE and EGCG as an option for treating cancer and its side effects. Nonetheless, further studies are required to elucidate their action mechanism on clotting, cell death and angiogenesis.
Funder
JBS Fund for the Amazon
Research Support Foundation of the State of Amapá-FAPEAP
Federal University of Rio Grande do Norte
Subject
Process Chemistry and Technology,Chemical Engineering (miscellaneous),Bioengineering
Reference72 articles.
1. Hagberg, C., Gottumukkala, V., Riedel, B., Nates, J., and Buggy, D. (2023). Cancer Epidemiology, Prevention, and Survivorship, Elsevier.
2. (2022, November 10). Cancer. Available online: https://www.who.int/news-room/fact-sheets/detail/cancer.
3. Cancer statistics, 2022;Siegel;CA Cancer, J. Clin.,2022
4. Current state of melanoma diagnosis and treatment;Davis;Cancer Biol. Ther.,2019
5. Saginala, K., Barsouk, A., Aluru, J.S., Rawla, P., and Barsouk, A. (2021). Epidemiology of melanoma. Med. Sci., 9.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献