Urine Sampling Protocol Recommendations for Reliable Determination of Total Urinary Luteinizing Hormone Immunoreactivity in the Pediatric Population

Author:

Demir And1ORCID,Aydin Adem2ORCID,Büyükgebiz Atilla3ORCID

Affiliation:

1. Pediatric Research Center, New Children’s Hospital, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland

2. Department of Pediatrics, Faculty of Medicine, Dokuz Eylül University, Izmir 35340, Türkiye

3. Department of Pediatrics, Division of Pediatric Endocrinology, Demiroğlu Bilim University, Istanbul 34394, Türkiye

Abstract

This study investigates day-to-day variations in urinary luteinizing hormone (U-LH) concentrations in children, focusing on potential minimization or correction methods. 95 children and adolescents (51 boys, 44 girls, ages 5–17) provided daytime and evening urine samples for U-LH determinations over three consecutive days. No consistent day-to-day differences in U-LH levels were observed, although random variations, particularly in adolescents aged 13 or older, were noted. The net inter-assay CV% for U-LH changes over three days showed high variability, averaging 24.6% to 28.0% for boys and 21.6% to 27.3% for girls, independent of sex, collection time, or U-LH level. To reliably determine total urinary luteinizing hormone immunoreactivity in the pediatric population, it is advisable to collect multiple first-morning voided samples for at least three consecutive days as an interim solution, pending the development of a standardized protocol or correction method for varying urine composition. Strict adherence, especially for adolescents aged 13 or older, is vital.

Funder

Finnish Medical Foundation and Päivikki and Sakari Sohlberg Foundation

University of Helsinki

Publisher

MDPI AG

Subject

Pediatrics, Perinatology and Child Health

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