Abstract
In vitro mutagenesis offers a feasible approach for developing new orchid cultivars through genetic manipulation. In the present study, protocorm-like bodies (PLBs) were exposed to gamma rays (10, 20, 40, 60, 80 Gy) to study in vitro growth responses and induction of mutants in Dendrobium ‘Emma White’. Both proliferation and regeneration of PLBs decreased progressively with increasing doses, except for a significantly enhanced growth response at 10 Gy. The optimal dose of gamma radiation for mutagenesis was found in the range 10 to 25 Gy based on the growth reduction curve. Analysis using a high-throughput cell analyzer revealed a significant reduction in nuclear DNA content at > 40 Gy doses. At 10 Gy treatment, the growth attributes, such as root length, plant height and leaf number, were significantly increased by 36%, 26% and 20%, respectively, compared to the control. This increase was significant over other tested doses as well. Testing of random amplified polymorphic DNA markers revealed the presence of detectable polymorphism among gamma mutant plantlets with a polymorphism information content value at 0.41. The gamma-ray-induced earliness in flower development was observed within 294 days post ex vitro growth of 10 Gy mutant compared to the control plants flowered after 959 days. Our results highlight the significance of gamma radiation in inducing enhanced growth, morphological variations and early floral initiation in Dendrobium, providing a basic framework for mutation breeding and improvement of orchids.
Funder
Atomic Energy (DAE), Board of Research in Nuclear Sciences (BRNS), Government of India
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference78 articles.
1. Dendrobium: Sources of Active Ingredients to Treat Age-Related Pathologies;Aging Dis.,2017
2. A Review on Molecular Systematic of the Genus Dendrobium Sw;Acta Biol. Malays.,2013
3. A Review of the Trade in Orchids and Its Implications for Conservation;Bot. J. Linn. Soc.,2018
4. Current Status of Orchid Production in Thailand;Acta Hortic.,2015
5. Baker, M.L., and Baker, C.O. (1996). Orchid Species Culture: Dendrobium, Timber Press.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献