The Effect of Leaf Wounding on Basil Plants of Different Developmental Stages

Abstract

Leaf wounding is a common stress that triggers a great number of plant mechanisms, while the overall plant status and age could also be critical for these mechanisms. However, there are not sufficient data about plants’ physiological responses after leaf wounding that has been imposed at different developmental stages. In this study, physiological parameters, such as photosynthesis, transpiration, and stomatal conductance, as well as the chlorophyll and anthocyanin leaf contents, of Ocimum basilicum var. minimum L. plants were measured for seven days on wounded plants during three different developmental stages (vegetative, budding, and flowering). All of the measurements were conducted on control and wounded plants, while on the latter they were conducted on both wounded and intact leaves. The physiological parameters mentioned above revealed a remarkable decrease in wounded leaves of the budding and flowering plants, while they seemed to be only partially affected on the leaves of vegetative plants. The physiological parameters’ decrease was not only an immediate plant response that was observed 1–2 h after wounding, but, in general, it was constant (during the seven days of treatments) and diurnal (from 8 a.m. to 8 p.m.). The wounded leaves revealed an immediate and constant anthocyanin content decrease during all of the developmental stages, while the corresponding chlorophyll decrease was mainly evident in the flowering plants. Regarding the intact leaves, they exhibited, in general, a similar profile to that of the control ones. The results above reveal that at the vegetative stage, basil plants are more tolerant to leaf wounding than those at the budding and flowering stages, implying that the plant’s response to wounding is a phenomenon that depends on the plant’s developmental stage.