Effects of Low-Temperature Stress during the Anther Differentiation Period on Winter Wheat Photosynthetic Performance and Spike-Setting Characteristics

Abstract

Climate change has caused frequent extreme low-temperature events to threaten global food security. Spring low-temperature stress is one of the major limiting factors for high and stable yields of wheat. We used two wheat varieties differing in spring cold-sensitivity (cold-tolerant variety Yannong 19 and cold-sensitive variety Xinmai 26) to examine the effects of low-temperature stress during the anther differentiation period on wheat photosynthetic performance and spike-setting characteristics. Low-temperature stress was simulated in a climate box at −2 °C, 0 °C or 2 °C (night) and 15 °C (day) for 24 h, 48 h or 72 h. With the extension of the treatment time and the decrease of temperature, the photosynthetic rate, stomatal conductance and transpiration rate of wheat leaves gradually decreased. All treatments except −2 °C for 72 h recovered slowly within 7–15 days after treatment. Low-temperature stress greatly reduced grains per spikelet, 1000-grain weight and yield per plant. By analyzing the spikelets in different stalk locations (upper, middle and lower), we found that the number of upper spikelets was significantly less than lower and middle spikelets after low-temperature stress. The sterile grain of upper spikelets (Xinmai 26, for example) can reach 100% at −2 °C for 48 h and 72 h, and the yield loss rate was 90.52% at 2 °C for 24 h, which was much higher than for the lower spikelets (60.73%) and middle spikelets (50.94%). Overall, these findings suggest that low-temperature stress during the anther differentiation period alters the photosynthetic activity involved in the accumulation of dry matter in wheat, which leads to delaying young spike growth, especially for upper spikelets, and ultimately in a decrease in yield.