Effect of Pb-Contaminated Water on Ludwigia stolonifera (Guill. & Perr.) P.H. Raven Physiology and Phytoremediation Performance

Abstract

A laboratory experiment was led to examine the lead bioaccumulation capacity of Ludwigia stolonifera (Guill. & Perr.) exposed to various Pb concentrations (0, 10, 25, 50, and 100 mg/L) for 1, 3, 5, and 7 days. The lead accumulation increased as the metal concentrations in the solution increased and over time, to an extreme accretion of 6840 mg/kg DW(dry weight) at 100 mg/L of lead on the 10 days exposure. The proportion removal efficiency, translocation factor, and bioconcentration factor of the plant were assessed. The maximum bioconcentration factor values (1981.13) indicate that the plant was a Pb hyperaccumulator, and translocation factor values (1.85), which are >1, indicate fit of L. stolonifera for eliminating Pb in Pb-contaminated water. Photosynthetic pigments were decreased with increase of Pb concentration and time exposure. Total chlorophyll content and Chl a/b ratio lowered to between 46 and 62% at 100 mg/L Pb after 10 days exposure. Protein content and soluble carbohydrate indicated a similar trend, which showed the highest decrease (7.26 and 36.2 mg/g FW(fresh weight), respectively) at 100 mg/L of Pb after 10 days. The activity of the antioxidant enzymes superoxide dismutase, ascorbate, and peroxidase was increased significantly in comparison to the control. The results indicate that L. stolonifera is a newly recognized Pb hyperaccumulator (6840 mg/kg DW), but physiological status indicates that the plant is not tolerant to high Pb concentrations.