Microhabitat and Pollinator Differentiation Drive Reproductive Isolation between Two Sympatric Salvia Species (Lamiaceae)

Abstract

Evaluation of multiple barriers contributing to reproductive isolation between sympatric plant species is key to understanding the mechanism of their coexistence; however, such investigations in biodiversity hotspots are still rare. In this study, we investigated and compared geography, microhabitat, phenology, flora, and pollinators, in addition to pollen–pistil interactions, seed production, and seed germination of the closely related sympatric Salvia digitaloides and S. flava on Yulong Snow Mountain, Southwestern Yunnan, China. The geographic distribution of these species overlapped, but their adaptation to physical and chemical properties of soil microhabitats differed. They shared the same flowering time but differed in flower size, style length, nectar volume, sugar concentration, and flower longevity. Both species shared bumblebees as effective pollinators, but flower constancy for the two species was relatively strong. Pollen tube growth, seed production, and seed germination were lower in interspecific than in intraspecific crosses. Our study suggested that microhabitat and pollinator isolation acted as the most important isolating barriers in maintaining the coexistence of the two Salvia species. Our study also highlighted that post-pollination barriers play an important role in preventing the gene flow between these two Salvia species.