Biogeographic Patterns of Leaf Element Stoichiometry of Stellera chamaejasme L. in Degraded Grasslands on Inner Mongolia Plateau and Qinghai-Tibetan Plateau

Abstract

Plant leaf stoichiometry reflects its adaptation to the environment. Leaf stoichiometry variations across different environments have been extensively studied in grassland plants, but little is known about intraspecific leaf stoichiometry, especially for widely distributed species, such as Stellera chamaejasme L. We present the first study on the leaf stoichiometry of S. chamaejasme and evaluate its relationships with environmental variables. S. chamaejasme leaf and soil samples from 29 invaded sites in the two plateaus of distinct environments [the Inner Mongolian Plateau (IM) and Qinghai-Tibet Plateau (QT)] in Northern China were collected. Leaf C, N, P, and K and their stoichiometric ratios, and soil physicochemical properties were determined and compared with climate information from each sampling site. The results showed that mean leaf C, N, P, and K concentrations were 498.60, 19.95, 2.15, and 6.57 g kg−1; the average C:N, C:P, N:P, N:K and K:P ratios were 25.20, 245.57, 9.81, 3.13, and 3.21, respectively. The N:P:K-ratios in S. chamaejasme leaf might imply that its growth is restricted by K- or K+N. Moreover, the soil physicochemical properties in the S. chamaejasme-infested areas varied remarkably, and few significant correlations between S. chamaejasme leaf ecological stoichiometry and soil physicochemical properties were observed. These indicate the nutrient concentrations and stoichiometry of S. chamaejasme tend to be insensitive to variations in the soil nutrient availability, resulting in their broad distributions in China’s grasslands. Besides, different homeostasis strength of the C, N, K, and their ratios in S. chamaejasme leaves across all sites were observed, which means S. chamaejasme could be more conservative in their use of nutrients improving their adaptation to diverse conditions. Moreover, the leaf C and N contents of S. chamaejasm were unaffected by any climate factors. However, the correlation between leaf P content and climate factors was significant only in IM, while the leaf K happened to be significant in QT. Besides, MAP or MAT contribution was stronger in the leaf elements than soil by using mixed effects models, which illustrated once more the relatively weak effect of the soil physicochemical properties on the leaf elements. Finally, partial least squares path modeling suggested that leaf P or K contents were affected by different mechanisms in QT and IM regions, suggesting that S. chamaejasme can adapt to changing environments by adjusting its relationships with the climate or soil factors to improve its survival opportunities in degraded grasslands.