The Phytoremediation Potential and Physiological Adaptive Response of Tamarix tetrandra Pall. Ex M. Bieb. during the Restoration of Chronosequence Fly Ash Deposits

Abstract

The challenging process of identifying and selecting plant species suited to the phytoremediation of fly ash (FA) dumps involves studying their functional properties and physiological response to a deficit of essential elements and toxicity from heavy metal(loid)-induced oxidative stress. We hypothesised that Tamarix tetrandra has high potential to be used for the phytoremediation of FA deposit sites thanks to its secretion strategy and antioxidative system. In this study, this hypothesis was examined by determining the bioconcentration and translocation factors for As, B, Cr, Cu, Mn, Ni, Se and Zn at the FA disposal lagoons at the ‘Nikola Tesla A’ thermal power plant in Obrenovac, Serbia, three (lagoon L1) and eleven (lagoon L2) years after the phytoremediation process had begun, and by measuring parameters of photosynthetic efficiency and chlorophyll concentration, non-enzymatic antioxidant defence (carotenoids, anthocyanins and phenolics), oxidative stress (concentration of malondialdehyde—MDA) and total antioxidant capacity to neutralise DPPH free radical activity. Tamarisk not only showed the ability to phytostabilise As, Cr and Ni and to accumulate low-availability Mn, Zn and Cu, but also the potential to maintain the structural and functional integrity of cell membranes and stable vitality at L1 under multiple stress conditions due to the high synthesis of phenols and tolerance to increased salinity. However, toxic concentrations of B and Se in leaves induced oxidative stress in tamarisk at L2 (reflected in higher MDA content and lower vitality) and also decreased the synthesis of chlorophyll, carotenoids, anthocyanins and total antioxidant activity. In addition, the prooxidative behaviour of phenols in the presence of spin-stabilising metals from FA could also have resulted in their weaker antioxidant protection at L2. These findings indicate that the choice of tamarisk was justified, but only at the beginning of the phytoremediation process because its presence contributed to an improvement in the harsh conditions at FA deposit sites and the creation of more favourable conditions for new plant species. This knowledge can be of great importance when planning sustainable ash deposit site management worldwide.