Biophysical Equations and Pressure Probe Experiments to Determine Altered Growth Processes after Changes in Environment, Development, and Mutations

Abstract

Expansive growth is a culmination of many biological processes. It is fundamental to volume growth, development, morphogenesis, sensory responses, and environmental responses of plants, fungi, and algae. Expansive growth of walled cells and plant tissue can be accurately described by a set of three global biophysical equations that model the biophysical processes of water uptake, wall deformation, and turgor pressure. Importantly, these biophysical equations have been validated with the results of pressure probe experiments. Here, a systematic method (scheme) is presented that iterates between analyses with the biophysical equations and experiments conducted with the pressure probe. This iterative scheme is used to determine altered growth processes for four cases; two after changes in the environment, one after a change in development, and another after changes by mutation. It is shown that this iterative scheme can identify which biophysical processes are changed, the magnitude of the changes, and their contribution to the change in expansive growth rate. Dimensionless numbers are employed to determine the magnitude of the changes in the biophysical processes. The biological meaning and implication of the biophysical variables in the biophysical equations are discussed. Further, additional sets of global biophysical equations are presented and discussed.