Rodlet Cells Provide First Line of Defense against Swimbladder Nematode and Intestinal Coccidian in Anguilla anguilla

Abstract

A subpopulation of 97 European eels, Anguilla anguilla, was obtained from a local consortium of Lake Trasimeno. The fish were examined for parasites in the swimbladder (SB) and intestine; the SBs of 66 (68%) of the 97 eels contained 480 adult specimens of the nematode Anguillicoloides crassus (Kuwahara, Niimi and Itagaki, 1974) and the intensity of infection ranged from 1 to 18 adult worms per SB (7.27 ± 0.43, mean ± SE). In heavily infected SB, the wall was thicker; upon excision, black-brownish adult nematodes were noticed inside the lumen. The infected SBs showed a papillose aspect of the epithelium and frequent erosion, inflammation, hemorrhages, and dilation of blood vessels. In parasitized SBs, mast cells and macrophages were encountered in the mucosal layer; in several heavily infected SB, rodlet cells were the sole fish immune cells noticed in the epithelium and in close contact with the A. crassus. With reference to the eel intestinal epithelium, in fifteen eels, two developmental stages of coccidian were close to clusters of rodlet cells, the coccidian induced erosion of the epithelium. The results of a panel of 12 antibodies in the European eel infected tissues will be presented.