Effects of LPS, Poly (I:C) and Edwardsiella tarda on the Expression Patterns of IL-17 Family Members and Their Receptors in Spotted Sea Bass (Lateolabrax maculatus)

Abstract

In fish, the immune system plays a crucial role in defending against pathogen-induced infections. The interleukin 17 (IL-17) family, which is a well-studied class of cytokines, serves as a key component of the immune response against external pathogens. In this research, four IL-17 ligands (IL-17A/F1, IL-17B, IL-17C and IL-17D) and one receptor (IL-17RB) genes were identified from spotted sea bass (Lateolabrax maculatus). Alignment analysis showed that the C-terminal region of IL-17 ligands in spotted sea bass was highly conserved. The expression of the IL-17 ligand and receptor genes differed in unstimulated tissues. To investigate the impact of various factors on the immune response of spotted sea bass, we assessed the effect of lipopolysaccharide (LPS), polyinosinic–polycytidylic acid [poly (I:C)] and Edwardsiella tarda treatment on the IL-17 ligands and receptor responses. Our results reveal that the expression of the IL-17 ligand and receptor transcripts is modulated by LPS, poly (I:C) and E. tarda, indicating their significant role in the immune system.