Editing the Melanocortin-4 Receptor Gene in Channel Catfish Using the CRISPR-Cas9 System

Author:

Khalil Karim123ORCID,Elaswad Ahmed14ORCID,Abdelrahman Hisham15ORCID,Michel Maximillian67ORCID,Chen Wenbiao6,Liu Shikai1,Odin Ramjie18,Ye Zhi1910ORCID,Drescher David111,Vo Khoi1,Bugg William S.112,Qin Guyu113,Yang Yujia1,Backenstose Nathan J. C.114,Liu Zhanjiang15ORCID,Cone Roger D.16,Dunham Rex1

Affiliation:

1. School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA

2. Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt

3. Department of Veterinary Medicine, College of Applied & Health Sciences, A’Sharqiyah University, Ibra 400, Oman

4. Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt

5. Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt

6. Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA

7. Institute of Zoology, Faculty of Mathematics and Natural Sciences, University of Cologne, 62 50674 Cologne, Germany

8. College of Fisheries, Mindanao State University-Maguindanao, Maguindanao 9601, Philippines

9. MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

10. Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, China

11. Muckleshoot Indian Tribe, Department of Fisheries 39015-A 172nd Ave SE, Auburn, WA 98092, USA

12. Department of Biological Sciences, University of Manitoba, Winnipeg, MB R3T 2M5, Canada

13. Division of Cardiovascular Medicine, Stanford University, Palo Alto, CA 94305, USA

14. Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14228, USA

15. Department of Biology, College of Arts and Sciences, Syracuse University, Syracuse, NY 13244, USA

16. Department of Molecular and Integrative Physiology, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USA

Abstract

The melanocortin-4 receptor (MC4R) plays a critical role in homeostasis and the regulation of body weight. Polymorphisms in the mc4r gene have been discovered and linked to growth, carcass composition, and meat quality traits. Therefore, we used the CRISPR-Cas9 system to target the mc4r gene in the most important freshwater aquaculture species in the USA, channel catfish, Ictalurus punctatus. Guide RNAs were designed to direct the Cas9 to the coding sequence of the channel catfish mc4r gene. gRNA(s)-Cas9 mixtures were delivered into one-cell embryos using electroporation and microinjection. For each treatment, the nature and rate of mutations were analyzed. Hatching and survival rates were calculated. The overall mutation rates were 30.6% and 66.7–90.6% for electroporation and microinjection, respectively. Mutated fish generated via electroporation or microinjection exhibited 38% and 20% improvement in body weight, respectively, when compared with the full-sib control. The mean feed conversion ratio of the mutants was 1.18 compared with 1.57 in the control fish. The improved growth and feed conversion indicate that the generation of mc4r-edited fish could economically benefit aquaculture production.

Funder

United States Department of Agriculture National Institute of Food and Agriculture

Publisher

MDPI AG

Subject

Ecology,Aquatic Science,Ecology, Evolution, Behavior and Systematics

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3