Attomole Electrochemical Detection of MicroRNAs Based on Surface-Initiated Enzymatic Polymerization Coupled with Copper Enhancement

Author:

Zhu Wenyuan123ORCID,Xu Yuzhi4ORCID,Zhang Yanfei1,Liu Si-Yang1ORCID,Dai Zong1,Zou Xiaoyong2

Affiliation:

1. Guangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen 518107, China

2. School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China

3. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China

4. Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518107, China

Abstract

The sensitive and effective detection of microRNAs (miRNAs) is of great significance since miRNAs have been proven to have undeniable importance in participating in many biological processes. Herein, we present a novel, sensitive, label-free electrochemical miRNA detection method. Three signal amplification techniques are incorporated in this method, including the efficient conjugate of primer-modified polystyrene spheres (PS) with magnetic beads (MBs) triggered by target miRNA, template-free surface-initiated enzymatic polymerization (SIEP) on the primers, and the use of copper ions in square wave voltammetry (SWV) for detecting acidically depurinated primers. Cooperating with the electrochemical approach, this method was able to achieve a detection limit of 120 aM. With an attomole level of sensitivity and easiness of manipulation, this novel method is suitable for miRNA routine detection in both research and clinical aspects.

Funder

the Guangdong Natural Science Foundation

the National Natural Science Foundation of China

Guangdong Science and Technology Plan Project Grant

Publisher

MDPI AG

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