De Novo Assembly, Characterization, and Comparative Transcriptome Analysis of Mature Male and Female Gonads of Rabbitfish (Siganus oramin) (Bloch & Schneider, 1801)-Reference-Cited by-同舟云学术

De Novo Assembly, Characterization, and Comparative Transcriptome Analysis of Mature Male and Female Gonads of Rabbitfish (Siganus oramin) (Bloch & Schneider, 1801)

Published:2024-04-30 Issue:9 Volume:14 Page:1346
ISSN:2076-2615
Container-title:Animals
language:en
Short-container-title:Animals

Author:

Huang Xiaolin¹²^ORCID,Huang Zhong¹²,Li Qiang³,Li Wenjun³,Han Chong³^ORCID,Yang Yukai¹²,Lin Heizhao¹²,Wu Qiaer¹²,Zhou Yanbo¹²

Affiliation:

1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

2. National Fishery Resources and Environment Dapeng Observation and Experimental Station, Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

3. School of Life Sciences, Guangzhou University, Guangzhou 510006, China

Abstract

The rabbitfish, Siganus oramin, is a commercially important table fish in southeastern China. However, there have been few studies on its gonad development and reproduction regulation. Comparative transcriptome analysis was first performed on adult male and female gonads of S. oramin. In total, 47,070 unigenes were successfully assembled and 22,737 unigenes were successfully annotated. Through comparative transcriptome analysis of male and female gonads, a total of 6722 differentially expressed genes were successfully identified, with 3528 upregulated genes and 3154 downregulated genes in the testes. In addition, 39 differentially expressed reproduction-related genes were identified. Finally, quantitative real-time PCR was used to validate the expression levels of several differentially expressed genes. These results provide important data for further studying the function of reproduction-related genes and the molecular mechanism regulating gonad development and reproduction in S. oramin.

Funder

Asian Cooperation Fund Program on Modern Fishery Cooperation between China and Other Coastal States of the South China Sea

Fund of the Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, P. R. China

Central Public-interest Scientific Institution Basal Research Fund

Hainan Engineering Research Center of Deep-Sea Aquaculture and Processing

Publisher

MDPI AG

Link

https://www.mdpi.com/2076-2615/14/9/1346/pdf

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