Affiliation:
1. Group for Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, 11000 Belgrade, Serbia
2. Group for Neuroendocrinology, Institute for Medical Research, University of Belgrade, 11000 Belgrade, Serbia
Abstract
Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.
Funder
Ministry of Education, Science and Technological Development, Republic of Serbia
Subject
Molecular Biology,Biochemistry
Reference48 articles.
1. Cecoro, G., Annunziata, M., Iuorio, M.T., Nastri, L., and Guida, L. (2020). Periodontitis, Low-Grade Inflammation and Systemic Health: A Scoping Review. Medicina, 56.
2. Role of salivary matrix metalloproteinase-8 (MMP-8) in chronic periodontitisdiagnosis;Gupta;Front. Med.,2015
3. Design of a multiple drug delivery system directed at periodontitis;Sundararaj;Biomaterials,2013
4. Clinical Therapeutic Effects of the Application of Doxycycline in the Treatment of Periodontal Disease;Spasovski;J. Med. Sci.,2016
5. The role of doxycycline as a matrix metalloproteinase inhibitor for the treatment of chronic wounds;Stechmiller;Biol. Res. Nurs.,2010