Switchable Polyacrylic Acid Polyelectrolyte Brushes for Surface Plasmon Resonance Applications

Abstract

Imaging wide-field surface plasmon resonance (SPR) microscopy sensors based on polyacrylic acid polyelectrolyte brushes (PAA PEBs) were designed to enhance the sensitivity of nano-object detection. The switching behavior of the PAA PEBs against changes in the pH values was investigated by analyzing the chemical, morphological, optical, and electrical properties. At pH ~1, the brushes collapse on the surface with the dominance of carboxylic groups (COOH). Upon the increase in the pH to nine, the switching process completes, and the brushes swell from dissociating most of the COOH groups and converting them into COO− groups. The domination of the negatively charged COO− groups increases the electrostatic repulsion in the polymer chains and stretches the brushes. The sensitivity of the SPR sensing device was investigated using a theoretical approach, as well as experimental measurements. The signal-to-noise ratio for a Au layer increases from six to eighteen after coating with PAA PEBs. In addition, the linewidth of the recorded image decreases from six pixels to five pixels by using the Au-PAA layers, which results from the enhanced spatial resolution of the recorded images. Coating a Au-layer with PAA PEBs enhances the sensitivity of the SPR sensing device, and improves the spatial resolution of the recorded image.