Candida krusei M4CK Produces a Bioemulsifier That Acts on Melaleuca Essential Oil and Aids in Its Antibacterial and Antibiofilm Activity
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Published:2023-11-30
Issue:12
Volume:12
Page:1686
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ISSN:2079-6382
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Container-title:Antibiotics
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language:en
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Short-container-title:Antibiotics
Author:
Araujo Jéssica Mayra Mendes12ORCID, Monteiro Joveliane Melo12, Silva Douglas Henrique dos Santos2ORCID, Veira Amanda Karoline2, Silva Maria Raimunda Chagas3, Ferraz Fernanda Avelino2, Braga Fábio H. Ramos3ORCID, Siqueira Ezequias Pessoa de4, Monteiro Andrea de Souza12
Affiliation:
1. Rede de Biodiversidade e Biotecnologia da Amazônia Legal, BIONORTE, Saint Louis 65055-310, Brazil 2. Laboratório de Microbiologia Aplicada, Universidade CEUMA, Saint Louis 65075-120, Brazil 3. Laboratório de Ciências do Ambiente, Universidade CEUMA, Saint Louis 65075-120, Brazil 4. Laboratório de Química de Produtos Naturais, Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Brazil
Abstract
Surface-active compounds (SACs) of microbial origin are an active group of biomolecules with potential use in the formulation of emulsions. In this sense, the present study aimed to isolate and select yeasts from fruits that could produce SACs for essential oil emulsions. The Candida krusei M4CK was isolated from the Byrsonima crassifolia fruit to make SACs. This emulsification activity (E24) was equal to or greater 50% in all carbon sources, such as olive oil, sunflower oil, kerosene, hexane, and hexadecane. E24 followed exponential growth according to the growth phase. The stability of emulsions was maintained over a wide range of temperatures, pH, and salinity. The OMBE4CK (melaleuca essential oil emulsion) had better and more significant inhibitory potential for biofilm reduction formation. In addition, bioemulsifier BE4CK alone on Escherichia coli and Pseudomonas aeruginosa biofilm showed few effective results, while there was a significant eradication for Staphylococcus aureus biofilms. The biofilms formed by S. aureus were eradicated in all concentrations of OMBE4CK. At the same time, the preformed biofilm by E. coli and P. aeruginosa were removed entirely at concentrations of 25 mg/mL, 12.5 mg/mL, and 6.25 mg/mL. The results show that the bioemulsifier BE4CK may represent a new potential for antibiofilm application.
Funder
Fundação de Amparo à Pesquisa e o Desenvolvimento Científico e Tecnológico do Maranhão Conselho Nacional de Desenvolvimento Científico e Tecnológico
Subject
Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology
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