Natural Products Biosynthesis by Streptomyces netropsis IMV Ac-5025 under Exogenous Sterol Action
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Published:2024-02-01
Issue:2
Volume:13
Page:146
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ISSN:2079-6382
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Container-title:Antibiotics
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language:en
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Short-container-title:Antibiotics
Author:
Loboda Mariia1ORCID, Biliavska Liudmyla1, Iutynska Galyna1, Newitt Jake2, Mariychuk Ruslan3ORCID
Affiliation:
1. Department of General and Soil Microbiology, D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Akademika Zabolotnoho Str., 154, 03143 Kyiv, Ukraine 2. Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK 3. Department of Ecology, Faculty of Humanities and Natural Science, University of Presov, 08001 Presov, Slovakia
Abstract
Streptomycetes are known as producers of bioactive substances, particularly antibiotics. Streptomyces netropsis IMV Ac-5025 simultaneously produces different classes of antibiotics, including polyene compounds, phytohormones, and sterols, but the metabolic pathways involved in their biosynthesis are largely understudied. The aim of this work was to explore the biosynthesis of polyene antibiotics, sterols, and phytohormones when the producer is cultivated in a nutrient medium supplemented with exogenous β-sitosterol. Gas chromatography and high-performance liquid chromatography were applied to analyze the spectrum of bioactive compounds. The obtained results demonstrated not only an increase in the accumulation of biomass but also polyene antibiotics, intracellular sterols, auxins, and cytokinins, when cultivating S. netropsis IMV Ac-5025 in a liquid medium with the addition of β-sitosterol. The amount of biomass raised 1.5–2-fold, whilst the sum of polyene antibiotics increased 4.5-fold, sterols’ sum (ergosterol, cholesterol, stigmasterol, β-sitosterol, and 24-epibrassinolide) by 2.9-fold, auxins’ sum (indole-3-acetic acid, indole-3-acetic acid hydrazide, indole-3-carbinol, indole-3-butyric acid, indole-3-carboxaldehyde, and indole-3-carboxylic acid) by 6-fold, and cytokinins’ sum (zeatin, isopentyladenine, zeatin riboside, and isopentenyladenosine) by 11-fold. Thus, we put forward the hypothesis that β-sitosterol plays a regulatory role in the network of biosynthetic reactions of S. netropsis IMV Ac-5025.
Subject
Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology
Reference52 articles.
1. Kong, D., Wang, X., Nie, J., and Niu, G. (2019). Regulation of Antibiotic Production by Signaling Molecules in Streptomyces. Front. Microbiol., 10. 2. The Role of Streptomyces Species in Controlling Plant Diseases: A Comprehensive Review;Australas. Plant Pathol.,2023 3. Streptomyces and Their Specialised Metabolites for Phytopathogen Control–Comparative In Vitro and in Planta Metabolic Approaches;Dow;Front. Plant Sci.,2023 4. Walsh, C., and Wencewicz, T. (2020). Antibiotics: Challenges, Mechanisms, Opportunities, John Wiley & Sons. 5. Haro-Reyes, T., Díaz-Peralta, L., Galván-Hernández, A., Rodríguez-López, A., Rodríguez-Fragoso, L., and Ortega-Blake, I. (2022). Polyene Antibiotics Physical Chemistry and Their Effect on Lipid Membranes; Impacting Biological Processes and Medical Applications. Membranes, 12.
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