Plasmon Resonance of Silver Nanoparticles as a Method of Increasing Their Antibacterial Action

Abstract

In this article, a series of silver-containing dressings are prepared by metal-vapor synthesis (MVS), and their antibacterial properties are investigated. The antibacterial activity of the dressings containing silver nanoparticles (AgNPs) against some Gram-positive, and Gram-negative microorganisms (Staphylococcus aureus, Staphylococcus haemolyticus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Moraxella spp.) has been determined. Based on the plasmon resonance frequency of these nanoparticles, the frequency of laser irradiation of the dressing was chosen. The gauze bandage examined showed pronounced antibacterial properties, especially to Staphylococcus aureus strain. When 470 nm laser radiation, with a power of 5 mW, was applied for 5 min, 4 h after inoculating the Petri dish, and placing a bandage containing silver nanoparticles on it, the antibacterial effect of the latter significantly increased—both against Gram-positive and Gram-negative microorganisms. The structure and chemical composition of the silver-containing nanocomposite were studied by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and extended X-ray absorption fine structure (EXAFS). The synthesized AgNPs demonstrate narrow and monomodal particle size distribution with an average size of 1.75 nm. Atoms of metal in Ag/bandage system are mainly in Ag0 state, and the oxidized atoms are in the form of Ag-Ag-O groups.