Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated from Dogs and Cats in South Korea
-
Published:2023-04-13
Issue:4
Volume:12
Page:745
-
ISSN:2079-6382
-
Container-title:Antibiotics
-
language:en
-
Short-container-title:Antibiotics
Author:
Choi Ji-Hyun1, Ali Md. Sekendar1, Moon Bo-Youn1, Kang Hee-Young1, Kim Su-Jeong1, Song Hyun-Ju1, Mechesso Abraham Fikru2, Moon Dong-Chan3ORCID, Lim Suk-Kyung1
Affiliation:
1. Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si 39660, Republic of Korea 2. Department of Pathology and Microbiology, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68198-5900, USA 3. Division of Antimicrobial Resistance Research, Center for Infectious Diseases Research, Korea Disease Control and Prevention Agency, Cheongju 28159, Republic of Korea
Abstract
Overall, 836 Escherichia coli isolates (695 isolates from dogs and 141 from cats) were recovered from the diarrhea, skin/ear, urine, and genitals of dogs and cats between 2018 and 2019. Cefovecin and enrofloxacin resistance were noted in 17.1% and 21.2% of E. coli isolates, respectively. The cefovecin and enrofloxacin resistance rates were higher in dog isolates (18.1% and 22.9%) compared with the rates in cat isolates (12.1%, 12.8%). Interestingly, resistance to both antimicrobials was noted in 10.8% (90/836) of the isolates, predominantly in isolates from dogs. blaCTX-M-14, blaCTX-M-15, and blaCMY-2 were the most frequent extended-spectrum β-lactamase/plasmid-mediated AmpC β-lactamase (ESBL/AmpC)- gene types. The co-existence of blaCTX-M andblaCMY-2 was noted in six E. coli isolates from dogs. Sequencing analysis demonstrated that S83L and D87N in gyrA and S80I in parC were the most frequent point mutations in the quinolone resistance-determining regions of the cefovecin and enrofloxacin-resistant isolates. A total of 11 isolates from dogs carried the plasmid-mediated quinolone resistance genes (six aac(6’)-Ib-cr, four qnrS, and one qnrB), while only two cat isolates carried the qnrS gene. Multilocus sequence typing of the cefovecin and enrofloxacin-resistant isolates revealed that sequence type (ST)131 E. coli carrying blaCTX-M-14 and blaCTX-M-15 genes and ST405 E. coli carrying blaCMY-2 gene were predominant among the isolated E. coli strains. The majority of the ESBL/AmpC-producing isolates displayed diverse pulsed-field gel electrophoresis profiles. This study demonstrated that third-generation cephalosporin- and fluoroquinolone-resistant E. coli were widely distributed in companion animals. The detection of the pandemic ST131 clone carrying blaCTX-M-14/15 in companion animals presented a public health threat.
Funder
Animal and Plant Quarantine Agency
Subject
Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology
Reference63 articles.
1. Are Escherichia coli Pathotypes Still Relevant in the Era of Whole-Genome Sequencing?;Holt;Front. Cell. Infect. Microbiol.,2016 2. Díaz-Jiménez, D., García-Meniño, I., Herrera, A., García, V., López-Beceiro, A.M., Alonso, M.P., Blanco, J., and Mora, A. (2020). Genomic Characterization of Escherichia coli Isolates Belonging to a New Hybrid AEPEC/ExPEC Pathotype O153: H10-A-ST10 Eae-Beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples. Antibiotics, 9. 3. Escherichia coli from Animal Reservoirs as a Potential Source of Human Extraintestinal Pathogenic E. coli;Garenaux;FEMS Immunol. Med. Microbiol.,2011 4. Multiple ESBL-Producing Escherichia coli Sequence Types Carrying Quinolone and Aminoglycoside Resistance Genes Circulating in Companion and Domestic Farm Animals in Mwanza, Tanzania, Harbor Commonly Occurring Plasmids;Seni;Front. Microbiol.,2016 5. The Prevalence of Plasmid-Mediated Quinolone Resistance Determinants among Clinical Isolates of ESBL or AmpC-Producing Escherichia coli from Chinese Pediatric Patients;Han;Microbiol. Immunol.,2010
|
|