Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms

Author:

Heydari Ali1,Kim Nick D.1ORCID,Biggs Patrick J.23ORCID,Horswell Jacqui1,Gielen Gerty J. H. P.4,Siggins Alma5,Taylor Matthew D.6ORCID,Bromhead Collette1ORCID,Palmer Barry R.1ORCID

Affiliation:

1. School of Health Sciences, Massey University, Wellington 6021, New Zealand

2. School of Natural Sciences, Massey University, Palmerston North 4410, New Zealand

3. School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand

4. Scion, Rotorua 3010, New Zealand

5. School of Biological and Chemical Sciences and Ryan Institute, University of Galway, H91 TK33 Galway, Ireland

6. Waikato Regional Council, Hamilton 3240, New Zealand

Abstract

Accumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels.

Funder

Massey University MURF

Publisher

MDPI AG

Subject

Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology

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