Evidence of Antibiotic Resistance and Virulence Factors in Environmental Isolates of Vibrio Species
-
Published:2023-06-16
Issue:6
Volume:12
Page:1062
-
ISSN:2079-6382
-
Container-title:Antibiotics
-
language:en
-
Short-container-title:Antibiotics
Author:
Pandey Rajkishor12ORCID, Sharma Simran3, Sinha Kislay Kumar1
Affiliation:
1. Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur 844102, Bihar, India 2. School of Medicine, University of Missouri, Columbia, MO 65211, USA 3. Department of Basic and Applied Sciences, National Institute of Food Technology Entrepreneurship & Management (NIFTEM), Kundli, Sonipat 131028, Haryana, India
Abstract
The outbreak of waterborne diseases such as cholera and non-cholera (vibriosis) is continuously increasing in the environment due to fecal and sewage discharge in water sources. Cholera and vibriosis are caused by different species of Vibrio genus which are responsible for acute diarrheal disease and soft tissue damage. Although incidences of cholera and vibriosis have been reported from the Vaishali district of Bihar, India, clinical or environmental strains have not been characterized in this region. Out of fifty environmental water samples, twelve different biochemical test results confirmed the presence of twenty Vibrio isolates. The isolates were found to belong to five different Vibrio species, namely V. proteolyticus, V. campbellii, V. nereis, V. cincinnatiensis, and V. harveyi. From the identified isolates, 65% and 45% isolates were found to be resistant to ampicillin and cephalexin, respectively. Additionally, two isolates were found to be resistant against six and four separately selected antibiotics. Furthermore, virulent hlyA and ompW genes were detected by PCR in two different isolates. Additionally, phage induction was also noticed in two different isolates which carry lysogenic phage in their genome. Overall, the results reported the identification of five different Vibrio species in environmental water samples. The isolates showed multiple antibacterial resistance, phage induction, and virulence gene profile in their genome.
Subject
Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology
Reference48 articles.
1. Muzembo, B.A., Kitahara, K., Debnath, A., Ohno, A., Okamoto, K., and Miyoshi, S.I. (2022). Cholera Outbreaks in India, 2011–2020: A Systematic Review. Int. J. Environ. Res. Public Health, 19. 2. Vibrio harveyi: A serious pathogen of fish and invertebrates in mariculture;Zhang;Mar. Life Sci. Technol.,2020 3. The emergence of Vibrio pathogens in Europe: Ecology, evolution, and pathogenesis (Paris, 11–12th March 2015);Wegner;Front. Microbiol.,2015 4. Non-cholera Vibrio species-currently still rare but growing danger of infection in the North Sea and the Baltic Sea;Brehm;Internist,2021 5. Vibrio spp. infections;Oliver;Nat. Rev. Dis. Prim.,2018
|
|