Characterization of Resistance and Virulence of Pasteurella multocida Isolated from Pet Cats in South China

Abstract

Pasteurella multocida, one of the major zoonotic pathogens, may cause localized cellulitis, bacteremia, septic shock, and other symptoms in people. Accidental bites or scratches in close contact between humans and cats are one of the causes of P. multocida infection in humans. The prevalence of diseases due to P. multocida in humans may be rising as a result of the popularity of cat cafes in China. However, the epidemiology of P. multocida in cat-themed cafes in South China is yet to be reported. P. multocida in 92 healthy cats from four cafes with pet-cat themes was examined in their tonsils and oral cavities. The antibiotic susceptibility of each isolate was evaluated by using the disk-susceptibility testing method. The 19 P. multocida were selected for whole-genome sequencing (WGS), together with the genome data of eight previously described clinical strains isolated from humans, and the analysis of antimicrobial resistance (AMR) determinants, capsular genotyping, MLST genotyping, and virulence gene profiles was carried out. The results showed that 48.91% of cats (45/90) were positive for P. multocida. All isolated P. multocida stains were highly resistant to erythromycin (15 μg) (100%) and nonsensitive to fluoroquinolones (5 μg) (37.38%), while they were all susceptible to penicillin (10 untis), tetracycline (30 μg), doxycycline (30 μg), and chloramphenicol (30 μg). According to WGS analysis, those with the gyrA resistance gene were all insensitive to fluoroquinolone antibiotics. Virulence gene profiles showed that the genes pfhA, nanH, and plpB were practically all present in cats compared to humans. To conclude, increased antibiotic resistance, along with virulence factors, complicates the treatment of P. multocida infection. Thus, clinical treatment for P. multocida infection should be performed more cautiously.