Cold Atmospheric Plasma Exerts Antimicrobial Effects in a 3D Skin Model of Cutaneous Candidiasis

Author:

Fink Sarah1,Fischer Michael2,Spange Sebastian3,Beier Oliver3,Horn Kerstin3,Tittelbach Jörg1ORCID,Wiegand Cornelia1

Affiliation:

1. Department of Dermatology, Jena University Hospital, Friedrich Schiller University, 07747 Jena, Germany

2. Institute of Micro- and Nanotechnologies, Ilmenau University of Technology, 98639 Ilmenau, Germany

3. Innovent e.V. Jena, 07745 Jena, Germany

Abstract

Cutaneous candidiasis is characterized by an overgrowth of Candida leading to skin inflammation and infection. Similar to bacteria, Candida can develop tolerance to common antifungal drugs. Cold atmospheric plasma (CAP), with its proven antimicrobial properties, offers a promising alternative to the prevailing methods. Because of plasma heterogeneity each new device must be tested individually for its effectiveness. Antimicrobial activity is usually studied using planktonic microorganisms or animal models, making it difficult to extrapolate the results to the human system. Therefore, a 3D skin model of cutaneous candidiasis for the antimicrobial testing of CAP was established. First, the reaction of the 3D-skin model to Candida infection was examined using various histological and molecular–biological methods. Infection with C. albicans resulted in increased expression and secretion of pro-inflammatory cytokines and augmented expression of antimicrobial peptides. Within 48 h, hyphal growth spread throughout the model and caused tissue damage. Second, the CAP treatment was employed. It was shown that CAP significantly reduced the spread of the yeast in the infected skin models as well as decreased the expression and secretion of the infection markers. The plasma device exhibited a high antifungal activity by completely inhibiting hyphal growth and reducing inflammation at the highest treatment duration.

Publisher

MDPI AG

Subject

Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology

Reference54 articles.

1. Cutaneous candidiasis—An evidence-based review of topical and systemic treatments to inform clinical practice;Taudorf;J. Eur. Acad. Dermatol. Venereol.,2019

2. Brogden, K.A., and Guthmiller, J.M. (2002). Polymicrobial Diseases, ASM Press.

3. Azole Antifungal Resistance in Candida albicans and Emerging Non-albicans Candida Species;Whaley;Front. Microbiol.,2016

4. Cold atmospheric pressure (physical) plasma in dermatology: Where are we today?;Friedman;Int. J. Dermatol.,2020

5. The emerging potential of cold atmospheric plasma in skin biology;Busco;Free Radic. Biol. Med.,2020

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3