Author:
Liu Zhiwei,Tong Zhaoyang,Wu Yuting,Liu Bing,Feng Shasha,Mu Xihui,Wang Jiang,Du Bin,Xu Jianjie,Liu Shuai
Abstract
A quantitative structure–activity relationship (QSAR) model for the structure and affinity of abrin aptamers was established. A higher affinity abrin aptamer based on the established QSAR model was screened by site-directed mutagenesis. The fluorescence quenching effect between magnetic microspheres and fluorescent molecules was studied for the first time. A new method for abrin detection based on the interaction between target molecules and fluorescently labeled aptamers on magnetic microspheres was developed, with the detection limit of 5 ng mL−1. This method can overcome the influence of complex environmental interferents in abrin detection and can meet the analysis requirements for simulated samples such as water, soil, and food.
Subject
General Materials Science