Acute Kaempferol Stimulation Induces AKT Phosphorylation in HepG2 Cells

Author:

Santana-Lima Beatriz1ORCID,Belaunde Lucas Humberto Zimmermann1,de Souza Karine Damaceno1,Rosa Matheus Elias2ORCID,de Carvalho Jose Eduardo3ORCID,Machado-Jr Joel4ORCID,Alonso-Vale Maria Isabel Cardoso14ORCID,Caseli Luciano25,Rando Daniela Gonçales Galasse16ORCID,Caperuto Luciana Chagas14ORCID

Affiliation:

1. Programa de Pós-Graduação em Biologia Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas—ICAQF, Universidade Federal de São Paulo, Diadema 09913-030, SP, Brazil

2. Programa de Pós-Graduação em Química—Ciência e Tecnologia da Sustentabilidade, Instituto de Ciências Ambientais, Químicas e Farmacêuticas—ICAQF, Universidade Federal de São Paulo, Diadema 09913-030, SP, Brazil

3. Departamento de Biologia e Ecologia Evolutiva, Instituto de Ciências Ambientais, Químicas e Farmacêuticas—ICAQF, Universidade Federal de São Paulo, Diadema 09913-030, SP, Brazil

4. Departamento de Ciências Biológicas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas—ICAQF, Universidade Federal de São Paulo, Diadema 09913-030, SP, Brazil

5. Departamento de Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas—ICAQF, Universidade Federal de São Paulo, Diadema 09913-030, SP, Brazil

6. Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas—ICAQF, Universidade Federal de São Paulo, Diadema 09913-030, SP, Brazil

Abstract

Type 2 diabetes mellitus (T2DM) stands as a prevalent global public health issue caused by deficiencies in the action of insulin and/or insulin production. In the liver, insulin plays an important role by inhibiting hepatic glucose production and stimulating glycogen storage, thereby contributing to blood glucose regulation. Kaempferitrin (KP) and kaempferol (KM), flavonoids found in Bauhinia forficata, exhibit insulin-mimetic properties, showing promise in managing T2DM. In this study, we aimed to assess the potential of these compounds in modulating the insulin signaling pathway and/or glucose metabolism. Cell viability assays confirmed the non-cytotoxic nature of both compounds toward HepG2 cells at the concentrations and times evaluated. Theoretical molecular docking studies revealed that KM had the best docking pose with the IR β subunit when compared to the KP. Moreover, Langmuir monolayer evaluation indicated molecular incorporation for both KM and KP. Specifically, KM exhibited the capability to increase AKT phosphorylation, a key kinase in insulin signaling, regardless of insulin receptor (IR) activation. Notably, KM showed an additional synergistic effect with insulin in activating AKT. In conclusion, our findings suggest the potential of KM as a promising compound for stimulating AKT activation, thereby influencing energy metabolism in T2DM.

Funder

the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil

São Paulo Research Foundation

Publisher

MDPI AG

Reference59 articles.

1. (2021). IDF Diabetes Atlas, International Diabetes Federation. [10th ed.].

2. Bauhinia forficata In Treatment Of Diabetes Mellitus: A Patent Review;Souza;Expert Opin. Ther. Pat.,2018

3. Chemical composition and biological potential of plants from the genus Bauhinia;Phytother. Res.,2009

4. Kaempferol, a flavonoid, ameliorates hyperglycemia by attenuating the key enzymes of carbohydrate metabolism in streptozotocin–induced experimental diabetic rats;Chandramohan;Prog. Nutr.,2020

5. Kaempferol alleviates insulin resistance via hepatic IKK/NF-κB signal in type 2 diabetic rats;Luo;Int. Immunopharmacol.,2015

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