Author:
Akuta Teruo,Maruyama Toshiaki,Sakuma Chiaki,Nakagawa Masataka,Tomioka Yui,Entzminger Kevin,Fleming Jonathan K.,Sato Ryo,Shibata Takashi,Kurosawa Yasunori,Okumura C. J.,Arakawa Tsutomu
Abstract
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescent protein showed functional state both on agarose gel and blotted membrane. Based on the combined procedures, we discovered conformation-specific monoclonal antibodies against PLXDC2 and SARS-CoV-2 spike protein.
Subject
Drug Discovery,Immunology,Immunology and Allergy
Cited by
9 articles.
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