Differential Expression of ATM, NF-KB, PINK1 and Foxo3a in Radiation-Induced Basal Cell Carcinoma
-
Published:2023-04-13
Issue:8
Volume:24
Page:7181
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Jenni Rim1, Chikhaoui Asma1, Nabouli Imen1, Zaouak Anissa2ORCID, Khanchel Fatma3, Hammami-Ghorbel Houda2, Yacoub-Youssef Houda1ORCID
Affiliation:
1. Laboratory of Biomedical Genomics and Oncogenetics (LR16IPT05), Institut Pasteur de Tunis, University Tunis El Manar, Tunis1002, Tunisia 2. Department of Dermatology, Habib Thameur Hospital (LR12SP03), Medicine Faculty, University Tunis El Manar, Tunis 1008, Tunisia 3. Anatomopathology Department, Habib Thameur Hospital (LR12SP03), Medicine Faculty, University Tunis El Manar, Tunis 1008, Tunisia
Abstract
Research in normal tissue radiobiology is in continuous progress to assess cellular response following ionizing radiation exposure especially linked to carcinogenesis risk. This was observed among patients with a history of radiotherapy of the scalp for ringworm who developed basal cell carcinoma (BCC). However, the involved mechanisms remain largely undefined. We performed a gene expression analysis of tumor biopsies and blood of radiation-induced BCC and sporadic patients using reverse transcription-quantitative PCR. Differences across groups were assessed by statistical analysis. Bioinformatic analyses were conducted using miRNet. We showed a significant overexpression of the FOXO3a, ATM, P65, TNF-α and PINK1 genes among radiation-induced BCCs compared to BCCs in sporadic patients. ATM expression level was correlated with FOXO3a. Based on receiver-operating characteristic curves, the differentially expressed genes could significantly discriminate between the two groups. Nevertheless, TNF-α and PINK1 blood expression showed no statistical differences between BCC groups. Bioinformatic analysis revealed that the candidate genes may represent putative targets for microRNAs in the skin. Our findings may yield clues as to the molecular mechanism involved in radiation-induced BCC, suggesting that deregulation of ATM-NF-kB signaling and PINK1 gene expression may contribute to BCC radiation carcinogenesis and that the analyzed genes could represent candidate radiation biomarkers associated with radiation-induced BCC.
Funder
Tunisian Ministry of Public Health, the Ministry of Higher Education and Scientific Research “Project Collaborative Interne”
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference97 articles.
1. Fania, L., Didona, D., Morese, R., Campana, I., Coco, V., Di Pietro, F.R., Ricci, F., Pallotta, S., Candi, E., and Abeni, D. (2020). Basal Cell Carcinoma: From Pathophysiology to Novel Therapeutic Approaches. Biomedicines, 8. 2. Global Cancer Observatory (2022, November 13). Estimated Number of New Cases in 2020, World, Both Sexes, All Ages. Available online: https://gco.iarc.fr/today/online-analysis-table. 3. Radiotherapy for benign disease; assessing the risk of radiation-induced cancer following exposure to intermediate dose radiation;McKeown;Br. J. Radiol.,2015 4. Radiation-induced skin carcinomas of the head and neck;Ron;Radiat. Res.,1991 5. Therapeutic ionizing radiation and the incidence of basal cell carcinoma and squamous cell carcinoma. The New Hampshire Skin Cancer Study Group;Lichter;Arch. Dermatol.,2000
|
|