Abstract
Cyclin B (CycB) plays essential roles in cell proliferation and promotes gonad development in many crustaceans. The goal of this study was to investigate the regulatory roles of this gene in the reproductive development of male oriental river prawns (Macrobrachium nipponense). A phylo-genetic tree analysis revealed that the protein sequence of Mn-CycB was most closely related to those of freshwater prawns, whereas the evolutionary distance from crabs was much longer. A quantitative PCR analysis showed that the expression of Mn-CycB was highest in the gonad of both male and female prawns compared to that in other tissues (p < 0.05), indicating that this gene may play essential roles in the regulation of both testis and ovary development in M. nipponense. In males, Mn-CycB expression in the testis and androgenic gland was higher during the reproductive season than during the non-reproductive season (p < 0.05), implying that CycB plays essential roles in the reproductive development of male M. nipponense. An RNA interference analysis revealed that the Mn-insulin-like androgenic gland hormone expression decreased as the Mn-CycB expression decreased, and that few sperm were detected 14 days after the dsCycB treatment, indicating that CycB positively affects testis development in M. nipponense. The results of this study highlight the functions of CycB in M. nipponense, and they can be applied to studies of male reproductive development in other crustacean species.
Funder
the Natural Science Foundation of Jiangsu Province
Jiangsu Agricultural Industry Technology System
China Agriculture Research System-48
New Cultivar Breeding Major Project of Jiangsu Province
Subject
Genetics (clinical),Genetics
Reference61 articles.
1. Current status and prospects of farming the giant river prawn (Macrobrachium rosenbergii) and the oriental river prawn (Macrobrachium nipponense) in china;Fu;Aquac. Res.,2012
2. Zhang, X.L., Cui, L.F., Li, S.M., Liu, X.Z., Han, X., and Jiang, K.Y. Bureau of Fisheries, Ministry of Agriculture, P.R.C. Fisheries economic statistics. China Fishery Yearbook, 2020.
3. Histological observation of gonadal development during post-larva in oriental river prawn, Macrobrachium nipponense;Jin;Chin. J. Fish.,2016
4. Identification of candidate genes of male sexual development from androgenic gland in Macrobrachium nipponense through performing long-reads and next generation transcriptome sequencing after eyestalk ablation;Jin;Sci. Rep.,2021
5. Transcriptome Profiling Analysis of the Testis After Eyestalk Ablation for Selection of the Candidate Genes Involved in the Male Sexual Development in Macrobrachium nipponense;Jin;Front. Genet.,2021
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