Abstract
Caladiums are promising colorful foliage plants due to their dazzling colors of the leaves, veins, stripes, and patches, which are often cultivated in pots or gardens as decorations. Four wild species, including C. bicolor, C. humboldtii, C. praetermissum, and C. lindenii, were employed in this study, where their chloroplast (cp) genomes were sequenced, assembled, and annotated via high-throughput sequencing. The whole cp genome size ranged from 162,776 bp to 168,888 bp, and the GC contents ranged from 35.09% to 35.91%. Compared with the single large copy (LSC) and single small copy (SSC) regions, more conserved sequences were identified in the inverted repeat regions (IR). We further analyzed the different region borders of nine species of Araceae and found the expansion or contraction of IR/SSC regions might account for the cp genome size variation. Totally, 131 genes were annotated in the cp genomes, including 86 protein-coding genes (PCGs), 37 tRNAs, and eight rRNAs. The effective number of codons (ENC) values and neutrality plot analyses provided the foundation that the natural selection pressure could greatly affect the codon preference. The GC3 content was significantly lower than that of GC1 and GC2, and codons ending with A/U had higher usage preferences. Finally, we conducted phylogenetic relationship analysis based on the chloroplast genomes of twelve species of Araceae, in which C. bicolor and C. humboldtii were grouped together, and C. lindenii was furthest from the other three Caladium species occupying a separate branch. These results will provide a basis for the identification, development, and utilization of Caladium germplasm.
Funder
Project of Collaborative Innovation Center of GDAAS
National Overseas Study Fund Program
Guangdong Science and Technology Planning Project
Subject
Genetics (clinical),Genetics
Reference67 articles.
1. Induction of tetraploids in ‘Red Flash’ Caladium using colchicine and oryzalin: Morphological, cytological, photosynthetic and chilling tolerance analysis;Sci. Hortic.,2020
2. Morphological, cytological and molecular marker analyses of ‘Tapestry’ Caladium variants reveal diverse genetic changes and enable association of leaf coloration pattern loci with molecular markers;Plant Cell Tiss. Org.,2020
3. Morphological, cytological, and pigment analysis of leaf color variants regenerated from long-term subcultured Caladium callus;In Vitro Cell Dev. Biol.-Plant,2021
4. A review of the Aroid Tribe Caladieae with the description of three new species of Caladium and seven new species of Syngonium (Araceae);Novon J. Bot. Nomencl.,2019
5. Evaluation of some neuropharmacological effects of Caladium bicolor aiton (Araceae) leaf extracts in mice;Metab. Brain Dis.,2019
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