Abstract
The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells.
Subject
Virology,Infectious Diseases
Reference73 articles.
1. Li, Z., Wang, J., Cheng, X., Hu, H., Guo, C., Huang, J., Chen, Z., and Lu, J. (2021). The Worldwide Seroprevalence of DENV, CHIKV and ZIKV Infection: A Systematic Review and Meta-Analysis. PLoS Negl. Trop. Dis., 15.
2. The Alphaviruses: Gene Expression, Replication, and Evolution;Strauss;Microbiol. Rev.,1994
3. Biology and Pathogenesis of Chikungunya Virus;Schwartz;Nat. Rev. Microbiol.,2010
4. Enhancement of Protein Expression by Alphavirus Replicons by Designing Self-Replicating Subgenomic RNAs;Kim;Proc. Natl. Acad. Sci. USA,2014
5. Alphavirus-Based Expression Vectors: Strategies and Applications;Frolov;Proc. Natl. Acad. Sci. USA,1996
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献